Journal: Heliyon

Article Title: AP-1 inhibitor induces ferroptosis via the PI3K/AKT pathway in multiple myeloma cells.

doi: 10.1016/j.heliyon.2024.e34397

Figure Lengend Snippet: Fig. 5. The PI3K/AKT signaling pathway is involved in AP-1 inhibitor-induced ferroptosis. (A) MM cells were exposed to T-5224 (20 μМ) for 48 h. The protein levels of p-AKT(Ser473), total AKT, p-PI3K (Tyr317), and total PI3K were assessed by western blotting. (B) MM cells were exposed to T- 5224 (20 μМ) for 48 h. The protein levels of Bcl2 and Bax in MM cells were assessed by western blotting. (C) MM cells were exposed to T-5224 in the presence or absence of Fer-1 (5 μM) for 48 h, after which the protein levels of p-AKT(Ser473), AKT, p-PI3K (Tyr317), and PI3K were assessed by western blotting. (D) MM cells were exposed to T-5224(20 μM) in the presence or absence of 740 Y–P (20 μM) for 48 h. The protein levels of GPX4 and SLC7A11 in MM cells were assessed by western blotting. β-actin served as the protein loading control. The results are presented as the mean ± standard deviation (SD).

Article Snippet: An anti-β-actin antibody was acquired from Proteintech.Anti-total-PI3K and anti-phosphoPI3KCA (Tyr317) antibodies were acquired from Bioss Co., Ltd. (Beijing, China).

Techniques: Western Blot, Control, Standard Deviation